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Immunohistochemical analysis of TRC105 effects on the liver fibrosis
Hamerníková, Veronika ; Němečková, Ivana (advisor) ; Svobodová, Zuzana (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department: Department of Biological and Medicinal Sciences Studijní obor: Pharmacy Author: Veronika Hamerníková Supervisor: RNDr. Ivana Němečková, Ph.D. Title of master thesis: Immunohistochemical analysis of TRC105 effects on the liver fibrosis The subject of this diploma thesis was the analysis of the expression of liver fibrosis biomarkers in mice liver. The aim of our work was to test the effect of the drug TRC105 (carotuximab) on a fibrotic liver model after 4 weeks of DDC (3,5-diethoxycarbonyl-1,4-dihydro-2,4,6-colidine) administration. Collagen deposition and expression of α-SMA, GFAP and endoglin were examined and compared. Animals were divided into 3 groups: control group (n=6), DDC group (n=6) and TRC105 group (n=6). The control group was fed a standard diet, while the DDC and the TRC105 groups were fed a diet with 0.1% DDC. Physiological solution was administered to the control and DDC groups while carotuximab was administered to the TRC105 group. The analysis of fibrosis biomarker expression was then performed on fixed liver sections. Collagen expression was evaluated by Picro-Sirius Red staining and α-SMA, GFAP and endoglin expression by immunohistochemistry with fluorescence imaging. Histological staining of collagen...
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